ABSTRACT
Shoot tip culture was explored for its potential to propagate and maintain selected plants or sectors of plants with individual worth and as a means of recovering plants from adventitious buds formed in tissue culture. Nutrient and carbohydrate concentration were found to affect shoot and root development. Medium containing standard concentrations of salts and carbohydrate and containing a low concentration of IAA was best for shoot growth and leaf development. Rooting was generally inhibited by exogenous growth regulators, but was enhanced in frequency by a lowered concentration of salts and carbohydrate. Also important was the type and concentration of exogenous growth regulators as well as the duration of exposure to growth regulators.
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