In October of 1983, six to eight week old calli derived from hypocotyl of 3 day old seedlings of Gossypium hirsutum, vars. C312, RQSX-1-1, C5110, P784,)303. ST213. T25 and T169 were found to contain globular to early heart-shaped embryos as determined by microscopic comparison to excised in-ovulo Gossypuim hirsutum embryos. Calli were placed into liquid culture where T25 and C312 formed exceptionally embryogenic cell suspensions. C312 was chosen as a model system for complete development of embryos to mature plant. Calli were initiated on Murashige and Skoog salts supplemented with 100 mg/l myo-inositol, 10 mg/l thiamine HCL, 1 mg/l nicotinic acid, 1 mg/l pyridoxine, 30 g/l sucrose, 0.1 mg/l 2,4-D, and 0.1 mg/l kinetin or 0.5 mg/l kinetin. Erlenmeyer flasks containing 50ml of liquid medium were inoculated with approximately 0.5g of callus tissue and rotated at 120 rpm. The culture was maintained by filtering through cheesecloth and returning a portion of the residue to basal medium. The filtrate containing single cells and small cell clumps (10-20 cells) was used to inoculate flasks containing the basal medium, without hormones and with addition of glutamine, for development of pro-embryonic units. Aliquots of the resulting embryogenic suspension were plated onto various solid media for further development and finally to Stewart's medium for equal growth of root and shoot meristems.