Bivalent pairing occurs for the 26 cotton chromosomes at metaphase. This is one chiasma per arm of these chromosomes that have median or submedian centromeres. That these are a maximum number and represent all that have occurred prometaphase is a presumption behind the older Stephens hypothesis. If so, clustering of loci and presence of interference should occur. That these are minimum number remaining is the basis for the newer Menzel hypothesis. Clustering of loci and translocation break points proximal and randomness distal should occur. Double recombination with and without interference should feature a linkage map. Localization is possible for some chromosomes and absent for others. Linkage groups III and V have multiple members in the long arms of 16 and 12, respectively. F₁ hybrids were made and tested reciprocally for double recombination and estimates of interference. Useful testers limited the testing an pauperate mutants at critical loci prevented their use i others. Through the pollen the R(1) ac Dw loci in distal 16L had double recombination without interference. Through the ovules, interference was present. Three chiasmata for 16L were indicated. Proximal the centromere in 12L, double recombination has been observed. A third chiasma distal the gl(2) locus indicated. Interference was not evaluated. However window effect, an increase of recombination for particular interval, is indicated for loci proximal the centromere. Clustering of loci for both LG III and V an multiple chiasmata are indicated but interference need further evaluation.