Cottonseed quality and production may be affected by changes in cultural practices at planting and harvesting and by the introduction of early maturing varieties. In 1988, experiments were conducted on six varieties of cotton; ST 506, ST 213, ST 825, DPL 20, DPL 50, DES 119, grown at Mississippi State in a skip raw planting pattern. At harvest, bolls were collected from selected positions on fruiting branches of the plants. For all six varieties of cotton, seed yield and quality were highest in bolls harvested from positions adjacent to the main stem on fruiting branches in the center of the plant. Quality of the seed was evaluated by fuzzy seed weight, kernel weight, oil content and free fatty acid content of the oil. Seed from bolls at other positions and branches were of poor quality due either to deterioration on the plant or immaturity.